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stempro ® msc sfm medium  (Thermo Fisher)


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    Structured Review

    Thermo Fisher stempro ® msc sfm medium
    Stempro ® Msc Sfm Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stempro%C2%AE-34+sfm+medium/stempro+msc+medium/pmc11979104-71-19-24
    Average 90 stars, based on 1 article reviews
    stempro ® msc sfm medium - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: Generation of human induced pluripotent stem cell line from peripheral blood of patient with lymphedema-distichiasis syndrome
    Article Snippet: 100 000 cells were transduced with Sendai vectors (CytoTuneTM-iPS 2.0 Sendai Reprogramming Kit, Thermo Fisher Scientific) according to the manufacturer’s protocol and seeded in StemPro®-34 SFM medium (Thermo Fisher Scientific), which was changed after 24 h. After 48 h cells were seeded on the plates coated with Geltrex (Thermo Fisher Scientific).

    Article Title: Generation of an integration-free induced pluripotent stem cell (iPSC) line (SDHI001-A) from a 65-year old adult mitral valve prolapse (MVP) patient.
    Article Snippet: These cells were then cultured on plates coated with recombinant human vitronectin (rhVTNN, Gibco) in StemPro®-34 SFM medium (Thermo Fisher Scientific) for 10 days. iPSCs were observed and then extended to passage 5–10 for characterization.

    Article Title: Generation of two induced pluripotent stem cell lines from Loeys-Dietz syndrome patients carrying heterologous mutation of TGFBR1
    Article Snippet: After isolation, the PBMCs were cultured in StemPro®-34 SFM medium (Thermo Fisher Scientific #10639011), which was supplemented with 100 ng/mL SCF (Peprotech #300-07), 100 ng/mL FLT3 (Thermo Fisher Scientific #PHC9414), and 20 ng/mL each of IL-3 (Peprotech #200-3), IL-6 (Thermo Fisher Scientific #PHC0063), and EPO (Thermo Fisher Scientific #PHC9631).

    Article Title: An iPSC model of fragile X syndrome reflects clinical phenotypes and reveals m 6 A-mediated epi-transcriptomic dysregulation underlying synaptic dysfunction
    Article Snippet: A total of 500,000 PBMCs were seeded in 24-well plates and cultured in PBMC medium [StemPro ® -34 SFM medium (ThermoFisher) supplemented with SCF (100ng/mL; Peprotech), FLT-3 (100ng/mL; Peprotech), IL-3 (20ng/mL; Peprotech), and IL-6 (20ng/mL; Peprotech)] for four days.

    Article Title: Generation of induced pluripotent stem cell line from a patient with long COVID
    Article Snippet: Culturing media consisted of StemPro®-34 SFM medium supplemented with specific growth factors and cytokines: 100 ng/mL FLT3 (ThermoFisher Scientific, #PHC9414), 100 ng/mL SCF (Peprotech, #300-07), 20 ng/mL IL-3 (Peprotech, #200-3), 20 ng/mL EPO (ThermoFisher Scientific, #PHC9631), and 20 ng/mL IL-6 (ThermoFisher Scientific, #PHC0063) and was used to support PBMCs as previously described [5].

    Article Title: Generation of two induced pluripotent stem cell lines from breast cancer patients carrying BRCA1 mutations
    Article Snippet: Reprogramming PBMCs were isolated from whole blood using Percoll® density gradient medium (GE Healthcare #17089109), washed with PBS, and cultured in StemPro®-34 SFM medium (Thermo Fisher Scientific #10639011) supplemented with 100 ng/mL SCF (Peprotech #300–07), 100 ng/mL FLT3 (Thermo Fisher Scientific #PHC9414), 20 ng/mL IL-3 (Peprotech #200–3), 20 ng/mL IL-6 (Thermo Fisher Scientific #PHC0063), and 20 ng/mL EPO (Thermo Fisher Scientific #PHC9631).

    Article Title: Generation of a human induced pluripotent stem cell lines (UKJi003-A) from a patient with Fabry disease and healthy donor (UKJi004-A)
    Article Snippet: The PBMCs were then incubated for three days in StemPro®-34 92 SFM medium (Thermo Fisher Scientific #10639011) daily supplemented with 10 ng/mL IL-6 (Thermo 93 Fisher Scientific #PHC 0064), 20 ng/mL TPO (Thermo Fisher Scientific, #PHC 9514), 100 ng/mL FLT3 94 (Thermo Fisher Scientific # PHC 9414), and 100 ng/mL SCF (Thermo Fisher Scientific #PHC 2115) for 95 enrichment.

    Article Title: Generation of human induced pluripotent stem cell lines UKJi001-A and UKJi006-A from patients with heterozygous mutation in the PKP2 gene.
    Article Snippet: One of the main signs we do not know enough about arrhythmogenic right ventricular dysplasia 9/cardiomyopathy (ARVCD9, OMIM #609040, autosomal dominant) is the lack of early markers and therapeutic alternatives.. To better study disease pathways in vitro, we generated human induced pluripotent stem cell (hiPSC) lines from the father (UKJi006-A) and son (UKJi001-A), who both shared the same heterozygous mutation in the PKP2 gene (OMIM *602861).. While the father had a clinical diagnosis of ARVC, the son lacked the ARVC phenotype.

    Cell Culture:

    Article Title: Generation of human induced pluripotent stem cell line from peripheral blood of patient with lymphedema-distichiasis syndrome
    Article Snippet: 100 000 cells were transduced with Sendai vectors (CytoTuneTM-iPS 2.0 Sendai Reprogramming Kit, Thermo Fisher Scientific) according to the manufacturer’s protocol and seeded in StemPro®-34 SFM medium (Thermo Fisher Scientific), which was changed after 24 h. After 48 h cells were seeded on the plates coated with Geltrex (Thermo Fisher Scientific).

    Article Title: Generation of an integration-free induced pluripotent stem cell (iPSC) line (SDHI001-A) from a 65-year old adult mitral valve prolapse (MVP) patient.
    Article Snippet: These cells were then cultured on plates coated with recombinant human vitronectin (rhVTNN, Gibco) in StemPro®-34 SFM medium (Thermo Fisher Scientific) for 10 days. iPSCs were observed and then extended to passage 5–10 for characterization.

    Article Title: Generation of two induced pluripotent stem cell lines from Loeys-Dietz syndrome patients carrying heterologous mutation of TGFBR1
    Article Snippet: After isolation, the PBMCs were cultured in StemPro®-34 SFM medium (Thermo Fisher Scientific #10639011), which was supplemented with 100 ng/mL SCF (Peprotech #300-07), 100 ng/mL FLT3 (Thermo Fisher Scientific #PHC9414), and 20 ng/mL each of IL-3 (Peprotech #200-3), IL-6 (Thermo Fisher Scientific #PHC0063), and EPO (Thermo Fisher Scientific #PHC9631).

    Article Title: An iPSC model of fragile X syndrome reflects clinical phenotypes and reveals m 6 A-mediated epi-transcriptomic dysregulation underlying synaptic dysfunction
    Article Snippet: A total of 500,000 PBMCs were seeded in 24-well plates and cultured in PBMC medium [StemPro ® -34 SFM medium (ThermoFisher) supplemented with SCF (100ng/mL; Peprotech), FLT-3 (100ng/mL; Peprotech), IL-3 (20ng/mL; Peprotech), and IL-6 (20ng/mL; Peprotech)] for four days.

    Article Title: Generation of induced pluripotent stem cell line from a patient with long COVID
    Article Snippet: Culturing media consisted of StemPro®-34 SFM medium supplemented with specific growth factors and cytokines: 100 ng/mL FLT3 (ThermoFisher Scientific, #PHC9414), 100 ng/mL SCF (Peprotech, #300-07), 20 ng/mL IL-3 (Peprotech, #200-3), 20 ng/mL EPO (ThermoFisher Scientific, #PHC9631), and 20 ng/mL IL-6 (ThermoFisher Scientific, #PHC0063) and was used to support PBMCs as previously described [5].

    Article Title: Generation of two induced pluripotent stem cell lines from breast cancer patients carrying BRCA1 mutations
    Article Snippet: Reprogramming PBMCs were isolated from whole blood using Percoll® density gradient medium (GE Healthcare #17089109), washed with PBS, and cultured in StemPro®-34 SFM medium (Thermo Fisher Scientific #10639011) supplemented with 100 ng/mL SCF (Peprotech #300–07), 100 ng/mL FLT3 (Thermo Fisher Scientific #PHC9414), 20 ng/mL IL-3 (Peprotech #200–3), 20 ng/mL IL-6 (Thermo Fisher Scientific #PHC0063), and 20 ng/mL EPO (Thermo Fisher Scientific #PHC9631).

    Article Title: Generation of a human induced pluripotent stem cell lines (UKJi003-A) from a patient with Fabry disease and healthy donor (UKJi004-A)
    Article Snippet: The PBMCs were then incubated for three days in StemPro®-34 92 SFM medium (Thermo Fisher Scientific #10639011) daily supplemented with 10 ng/mL IL-6 (Thermo 93 Fisher Scientific #PHC 0064), 20 ng/mL TPO (Thermo Fisher Scientific, #PHC 9514), 100 ng/mL FLT3 94 (Thermo Fisher Scientific # PHC 9414), and 100 ng/mL SCF (Thermo Fisher Scientific #PHC 2115) for 95 enrichment.

    Article Title: Generation of human induced pluripotent stem cell lines UKJi001-A and UKJi006-A from patients with heterozygous mutation in the PKP2 gene.
    Article Snippet: One of the main signs we do not know enough about arrhythmogenic right ventricular dysplasia 9/cardiomyopathy (ARVCD9, OMIM #609040, autosomal dominant) is the lack of early markers and therapeutic alternatives.. To better study disease pathways in vitro, we generated human induced pluripotent stem cell (hiPSC) lines from the father (UKJi006-A) and son (UKJi001-A), who both shared the same heterozygous mutation in the PKP2 gene (OMIM *602861).. While the father had a clinical diagnosis of ARVC, the son lacked the ARVC phenotype.

    Recombinant:

    Article Title: Generation of human induced pluripotent stem cell line from peripheral blood of patient with lymphedema-distichiasis syndrome
    Article Snippet: 100 000 cells were transduced with Sendai vectors (CytoTuneTM-iPS 2.0 Sendai Reprogramming Kit, Thermo Fisher Scientific) according to the manufacturer’s protocol and seeded in StemPro®-34 SFM medium (Thermo Fisher Scientific), which was changed after 24 h. After 48 h cells were seeded on the plates coated with Geltrex (Thermo Fisher Scientific).

    Article Title: Generation of an integration-free induced pluripotent stem cell (iPSC) line (SDHI001-A) from a 65-year old adult mitral valve prolapse (MVP) patient.
    Article Snippet: These cells were then cultured on plates coated with recombinant human vitronectin (rhVTNN, Gibco) in StemPro®-34 SFM medium (Thermo Fisher Scientific) for 10 days. iPSCs were observed and then extended to passage 5–10 for characterization.

    Article Title: Generation of two induced pluripotent stem cell lines from Loeys-Dietz syndrome patients carrying heterologous mutation of TGFBR1
    Article Snippet: After isolation, the PBMCs were cultured in StemPro®-34 SFM medium (Thermo Fisher Scientific #10639011), which was supplemented with 100 ng/mL SCF (Peprotech #300-07), 100 ng/mL FLT3 (Thermo Fisher Scientific #PHC9414), and 20 ng/mL each of IL-3 (Peprotech #200-3), IL-6 (Thermo Fisher Scientific #PHC0063), and EPO (Thermo Fisher Scientific #PHC9631).

    Article Title: An iPSC model of fragile X syndrome reflects clinical phenotypes and reveals m 6 A-mediated epi-transcriptomic dysregulation underlying synaptic dysfunction
    Article Snippet: A total of 500,000 PBMCs were seeded in 24-well plates and cultured in PBMC medium [StemPro ® -34 SFM medium (ThermoFisher) supplemented with SCF (100ng/mL; Peprotech), FLT-3 (100ng/mL; Peprotech), IL-3 (20ng/mL; Peprotech), and IL-6 (20ng/mL; Peprotech)] for four days.

    Article Title: Generation of induced pluripotent stem cell line from a patient with long COVID
    Article Snippet: Culturing media consisted of StemPro®-34 SFM medium supplemented with specific growth factors and cytokines: 100 ng/mL FLT3 (ThermoFisher Scientific, #PHC9414), 100 ng/mL SCF (Peprotech, #300-07), 20 ng/mL IL-3 (Peprotech, #200-3), 20 ng/mL EPO (ThermoFisher Scientific, #PHC9631), and 20 ng/mL IL-6 (ThermoFisher Scientific, #PHC0063) and was used to support PBMCs as previously described [5].

    Article Title: Generation of two induced pluripotent stem cell lines from breast cancer patients carrying BRCA1 mutations
    Article Snippet: Reprogramming PBMCs were isolated from whole blood using Percoll® density gradient medium (GE Healthcare #17089109), washed with PBS, and cultured in StemPro®-34 SFM medium (Thermo Fisher Scientific #10639011) supplemented with 100 ng/mL SCF (Peprotech #300–07), 100 ng/mL FLT3 (Thermo Fisher Scientific #PHC9414), 20 ng/mL IL-3 (Peprotech #200–3), 20 ng/mL IL-6 (Thermo Fisher Scientific #PHC0063), and 20 ng/mL EPO (Thermo Fisher Scientific #PHC9631).

    Article Title: Generation of a human induced pluripotent stem cell lines (UKJi003-A) from a patient with Fabry disease and healthy donor (UKJi004-A)
    Article Snippet: The PBMCs were then incubated for three days in StemPro®-34 92 SFM medium (Thermo Fisher Scientific #10639011) daily supplemented with 10 ng/mL IL-6 (Thermo 93 Fisher Scientific #PHC 0064), 20 ng/mL TPO (Thermo Fisher Scientific, #PHC 9514), 100 ng/mL FLT3 94 (Thermo Fisher Scientific # PHC 9414), and 100 ng/mL SCF (Thermo Fisher Scientific #PHC 2115) for 95 enrichment.

    Article Title: Generation of human induced pluripotent stem cell lines UKJi001-A and UKJi006-A from patients with heterozygous mutation in the PKP2 gene.
    Article Snippet: One of the main signs we do not know enough about arrhythmogenic right ventricular dysplasia 9/cardiomyopathy (ARVCD9, OMIM #609040, autosomal dominant) is the lack of early markers and therapeutic alternatives.. To better study disease pathways in vitro, we generated human induced pluripotent stem cell (hiPSC) lines from the father (UKJi006-A) and son (UKJi001-A), who both shared the same heterozygous mutation in the PKP2 gene (OMIM *602861).. While the father had a clinical diagnosis of ARVC, the son lacked the ARVC phenotype.



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